Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis i in mouse oocytes

Title: Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis i in mouse oocytes
Creator: Holt, Janet E.; Lane, Simon I. R.; Jones, Keith T.
Relation: Mammalian Oocyte Regulation: Methods and Protocols p. 91-106
Relation: Methods in Molecular Biology: Methods and Protocols 957
Publisher Link: http://dx.doi.org/10.1007/978-1-62703-191-2-6
Publisher: Humana Press
Resource Type: book chapter
Date: 2013
Description: The first meiotic division of mammalian oocytes physiologically occurs in the ovary in the hours preceding ovulation. Fortunately, oocytes removed from their follicular environment will readily undergo this process in culture. Their large size, optical transparency, and efficiency in translating exogenous cRNA make mouse oocytes very amenable to study this process in detail using fluorescence imaging-based techniques. Here we describe the process of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example.
Subject: RNA; epifluorescence; fluorescent protein; oocyte; imaging; meiosis
Identifier: http://hdl.handle.net/1959.13/1321158
Identifier: uon:24275
Identifier: ISBN:9781627031905
Language: eng